Table 1.

Primers used in this study for the analysis of gene expression by qPCR.

Gene Name	Nucleotide Sequence of Forward Primer (5′ to 3′)	Nucleotide Sequence of Reverse Primer (5′ to 3′)
ACT1	TGGATTCCGGTGATGGTGTT	CGGCCAAATCGATTCTCAA
ADY2	TTTCAGCCTTCGCGTTGAC	CTTGCGCTCTCGCATTGA
ATP20	GGGTCTTCAACCACCAACTGTT	GGCTCTGCTTATAAAGGTTCGAAT
CIS1	CCCATCGGGTTAGTTTCAAAAA	GACATGCTACCCACTCTGCAATAG
COX2	TCGTTGTAACAGCTGCTGATGTT	CCAGGAGTAGCATCAACTTTAATACCT
GDH3	CACCGGGTTCGGCTTAGTT	GCCGTTTGTTGCATAATCGA
HEM2	TTCCGCTATTCATCTCCGATAATCCAG	ACAGACATCGCAAATAATATACAGTTCAGG
MGA1	ATGGGCAGTCCCGTCCATTACT	TCGCATCATGTTCACCGTGGGT
MMT1	GCGTTGTTTGCGGATGCTA	GCAAAGTCAACAAGTCAGAAACCA
SDH6	ACTTCACCACCATTGAACACTTGT	GGGTGTGAAAAGGTGGCAATT
SRX1	CCTGTGTTGGATCCTCAA	GGCATAATATAGCGTCTGTC
TAF10	ATATTCCAGGATCAGGTCTTCCGTAGC	GTAGTCTTCTCATTCTGTTGATGTTGTTGTTG

Primer design was performed using Primer Express software (Primer Express 3.0 Applied Biosystems). The primer ACT1 was previously reported by Beltran et al. [34], and those for HEM2 and TAF10 were previously reported by Teste et al. [35].