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. 2020 Oct 1;9(10):947. doi: 10.3390/antiox9100947

Table 1.

Primers used in this study for the analysis of gene expression by qPCR.

Gene Name Nucleotide Sequence of Forward Primer (5′ to 3′) Nucleotide Sequence of Reverse Primer (5′ to 3′)
ACT1 TGGATTCCGGTGATGGTGTT CGGCCAAATCGATTCTCAA
ADY2 TTTCAGCCTTCGCGTTGAC CTTGCGCTCTCGCATTGA
ATP20 GGGTCTTCAACCACCAACTGTT GGCTCTGCTTATAAAGGTTCGAAT
CIS1 CCCATCGGGTTAGTTTCAAAAA GACATGCTACCCACTCTGCAATAG
COX2 TCGTTGTAACAGCTGCTGATGTT CCAGGAGTAGCATCAACTTTAATACCT
GDH3 CACCGGGTTCGGCTTAGTT GCCGTTTGTTGCATAATCGA
HEM2 TTCCGCTATTCATCTCCGATAATCCAG ACAGACATCGCAAATAATATACAGTTCAGG
MGA1 ATGGGCAGTCCCGTCCATTACT TCGCATCATGTTCACCGTGGGT
MMT1 GCGTTGTTTGCGGATGCTA GCAAAGTCAACAAGTCAGAAACCA
SDH6 ACTTCACCACCATTGAACACTTGT GGGTGTGAAAAGGTGGCAATT
SRX1 CCTGTGTTGGATCCTCAA GGCATAATATAGCGTCTGTC
TAF10 ATATTCCAGGATCAGGTCTTCCGTAGC GTAGTCTTCTCATTCTGTTGATGTTGTTGTTG

Primer design was performed using Primer Express software (Primer Express 3.0 Applied Biosystems). The primer ACT1 was previously reported by Beltran et al. [34], and those for HEM2 and TAF10 were previously reported by Teste et al. [35].