The activity of APCs against L. mexicana. WT or C12Rx promastigotes (5 × 10⁶/ml, n = 3 independent treatment in triplicate) were cultured in the presence of medium alone (control) or different concentrations of the alkylphosphocholines APC12, APC14 and APC16 for 3 days at 25 °C. The effect of treatment on parasite survival was determined by determining the mean suppression in parasite growth for each experimental value compared to the mean control value. These data were then used to determine the mean IC₅₀ value using the Grafit software. The resistant index RI₅₀ was the quotient of the mean IC₅₀ values of the two strains with C12Rx and the WT being the numerator and denominator, respectively; CMC, critical micellar concentration.

Compound	IC50 μM (μg ml−1)		p value	RI50	CMC (μM)
	WT	C12Rx
APC12	0.19 ± 001b	189.58 ± 10.14b	p < 0.05	952.00	1000a
	(0.07 ± 0.03)	(66.64 ± 12.26)
APC14	0.72 ± 0.02b	130.96 ± 6.12a	p < 0.05	184.07	120a
	(0.27 ± 0.05)	(49.70 ± 7.23)
APC16	4.32 ± 0.09xb	70.92 ± 6.78a	p < 0.05	16.42	10a
	(1.76 ± 0.20)	(28.90 ± 9.97)

^aMicellar. See Anatrace product description sheet for respective APCs.

^bNo micellar requirement.