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. 2020 Oct 22;20(6):247. doi: 10.3892/etm.2020.9377

Figure 3.

Figure 3

miR-122 regulates docetaxel resistance of PCa cells possibly via PKM2 regulation. The upregulation efficiency of oePKM2 lentivirus was detected by (A) reverse transcription-quantitiative PCR and (B) western blotting. ***P<0.001 vs. vector. (C) The efficiency of miR-122 mimic transfection was detected. ***P<0.001 vs. NC. LNCaP/Docetaxel cells were treated with both oePKM2 lentivirus and miR-122 mimic. (D) Cell proliferation was detected at 0, 24, 48 and 72 h. **P<0.01 vs. NC and ##P<0.01 vs. vector + mimic. (E) Cell apoptosis was detected at 48 h. The ordinate of the histogram is the sum of early and late apoptosis. ***P<0.001 vs. NC and ###P<0.001 vs. vector + mimic. (F) Glucose uptake and (G) lactate production were detected. ***P<0.001 vs. NC and ###P<0.001 vs. vector + mimic. PKM2 (H) mRNA and (I) protein expression was detected. ***P<0.001 vs. NC and ###P<0.001 vs. vector + mimic. miR-122, microRNA-122; OD, optical density; NC, negative control; PI, propidium iodide.