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. 2020 Sep 6;12(1):1814666. doi: 10.1080/20002297.2020.1814666

Table 2.

Primers used for PCR and sequencing.

Number Primer description Primer Sequence (5ʹ to 3ʹ) Primer position Source Use
1 TM7 16S rRNA 580 Fwd AI71 AYTGGGCGTAAAGAGTTGC 563–580a Referenceg Identification 600 base 16S rRNA fragment PCR most taxa
2 TM7 16S rRNA 1177 Rev AI72 GACCTGACATCATCCCCTCCTTCC 1177–1200a Referenceh Identification 600 base 16S rRNA fragment PCR most taxa
3 TM7 16S rRNA 33 Fwd AJ02 ATCCTGGCTCAGGATKAA 16–33a This paper Identification full 1500 base 16S rRNA PCR most taxa
4 TM7 16S rRNA 1524 Rev AI85 AAGGAGGTAATCCATCCG 1524–1541a This paper Identification full 1500 base 16S rRNA PCR most taxa
5 TM7 Fingerprint 16S rRNA 1291 Fwd AI73 AGCAAATCRCAYCAAARC 1275–1291a This paper Fingerprint region 16S rRNA to tRNA PCR
6 TM7 Fingerprint tRNA Ala 30 Rev AI78 ACCCCCTGCTTGCAAAGCA 30–48b This paper Fingerprint region 16S rRNA to tRNA PCR
7 TM7 Fingerprint tRNA Ile 30 Rev AI79 ACCTCGTCATTATCAGTGA 30–48b This paper Fingerprint region 16S rRNA to tRNA PCR
8 TM7 Fingerprint tRNA Val 30 Rev AI80 CCCTCTCGGTGTAAACGA 30–47b This paper Fingerprint region 16S rRNA to tRNA PCR
9 TM7 Fingerprint tRNA Ala 42 Fwd AI81 GCACCTGCTTTGCAAGCA 25–42b This paper Fingerprint region tRNA to 23S rRNA PCR
10 TM7 Fingerprint tRNA Ile 42 Fwd AI82 GCGCGTCACTGATAATGA 25–42b This paper Fingerprint region tRNA to 23S rRNA PCR
11 TM7 Fingerprint tRNA Val 42 Fwd AI83 GCATCTCGTTTACACCGA 25–42b This paper Fingerprint region tRNA to 23S rRNA PCR
12 TM7 23S rRNA 53 Rev AJ26 GCAGTCTTCCACGTCCTT 53–70 c This paper Fingerprint region tRNA to 23S rRNA PCR
13 TM7 23S rRNA 192 Rev AJ27 CTACTAAGATGTTTCAGTTCA 192–212 c This paper Fingerprint region tRNA to 23S rRNA PCR
14 TM7 23S rRNA 639 Rev AJ28 CGGGGTTCTTTTCACCTT 639–656 c This paper Fingerprint region tRNA to 23S rRNA PCR
15 AC001 Fingerprint RNA methyltransferase A Fwd AJ06 GCGGAACTTGGTGAAGA 565,869–565885d This paper Differentiate Saccharibacteria HMT-955 strains AC001 and HB001
16 AC001 Fingerprint RNA methyltransferase A Rev AJ07 CGTTAGCTTTACTAATACCCA 565,285–565303d This paper Differentiate Saccharibacteria HMT-955 strains AC001 and HB001
17 HB001 Fingerprint RNA methyltransferase A Fwd AJ08 GCGGAGCTGAGTAAGGA within genee This paper Differentiate Saccharibacteria HMT-955 strains AC001 and HB001
18 HB001 Fingerprint RNA methyltransferase A Rev AJ09 CAGCATCTTTACTGATAGCTA within genee This paper Differentiate Saccharibacteria HMT-955 strains AC001 and HB001
19 Arachnia propionica Fingerprint Fwd AI86 GCTGAGCGTAACATGAGAT 16,066–16,084 f This paper Differentiate Arachnia propionica strains F0230 and F0700
20 Arachnia propionica Fingerprint Rev AI87 CCATGTACTGCAAGGAATGT 15,631–15,650 f This paper Differentiate Arachnia propionica strains F0230 and F0700
21 TM7 16S rRNA HMT-488-plus but not HMT-955 Fwd AJ30 TTCCACAATGGGCGAAAG 367–384a This paper Differentiate Saccharibacteria species HMT-488 from HMT-955
22 TM7 16S rRNA HMT-488-plus but not HMT-955 Rev AJ31 CGGGGCAGTCCAAGTA 1150–1165a This paper Differentiate Saccharibacteria species HMT-488 from HMT-955
23 TM7 16S rRNA HMT-955-plus but not HMT-488 Fwd AJ32 TTCCACAATGGGGGCAAC 367–384a This paper Differentiate Saccharibacteria species HMT-488 from HMT-955
24 TM7 16S rRNA HMT-955-plus but not HMT-488 Rev AJ33 CCGGGGCAGTCTGAATA 1150–1166a This paper Differentiate Saccharibacteria species HMT-488 from HMT-955

aPosition in 16S rRNA as aligned to E. coli,bposition in tRNA, cposition in 23S rRNA alignment for Saccharibacteria, dposition in genome CP040003.1, ewaiting GenBank annotation, fposition in genome CP040007.1, gHugenholtz et al 2001, and hBrining et al 2003.