Figure 2.

In vitro cytotoxicity and internalization of PSPps. (A) Relative cell viabilities of HEK-293T, CRL-5802, NCI-520 and A549 cells exposed to PSPps at various concentrations. (B) Flow-cytometry diagrams of A549 cells exposed to rhodamine 123 (R123)-linked PSPps at 250 µg⋅mL⁻¹ for 1h and 4h incubation. (C) Flow-cytometry diagrams of A549 cells exposed to R123-linked PSPps at 250 µg⋅mL⁻¹ for 4h incubation after the cells were pretreated with different chemical inhibitors: 10 µg⋅mL⁻¹ chlorpromazine, 200 µM genistein and 50 nM wortmannin for 30 min.