Fig 2.

The three sex chromosomes of X. tropicalis can be crossed in six ways to produce offspring with different types of sex-linkage and/or skewed offspring sex ratios (left). Crosses on the left that are not shaded are expected to have male-specific SNPs passed from father to all sons in the male-specific portion of the Y chromosome. We generated three laboratory families from west and east Ghana for RRGS and RNAseq analyses (right). For the RNAseq analysis (Family 3), offspring were analyzed from a cross between the father and a daughter from Family 2 (indicated with arrows). On the right, putative sex chromosome genotypes described in main text are in parentheses with a question mark indicating either a W or a Z chromosome.