Extended Data Fig. 6. GUIDE-seq dsODN tag independent indel frequencies are strongly correlated with tag integration frequencies.

Comparison of standard targeted sequencing and rhAMPSeq, a multiplex targeted sequencing method used in our study to validate on- and off-target site mutations. Scatterplots of indel mutation frequencies (top) and tag integration frequencies (bottom), between standard amplicon sequencing and rhAMPSeq, for sgRNAs targeted against CTLA4 site 9 and TRAC site 2 (See Methods). b, Scatterplots showing correlation between indel frequencies (in cells edited with Cas9 RNPs and no dsODN tag) and tag integration frequencies (in cells edited with Cas9 RNP and dsODN tag) at on- and off-target sites measured by targeted amplicon sequencing. (a-b) Correlation between two samples was calculated using Pearson’s correlation coefficient.