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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Nat Biotechnol. 2020 Jun 15;38(11):1317–1327. doi: 10.1038/s41587-020-0555-7

Extended Data Fig. 7. Targeted tag sequencing validation of CHANGE-seq detected off-target sites.

Extended Data Fig. 7

Targeted tag integration frequencies evaluated by standard targeted sequencing (triangle shape) and or rhAMPSeq (circle shape) (See Methods) at on- and off-target sites detected by both GUIDE-seq and CHANGE-seq, or detected by CHANGE-seq only (classes A-D), for sgRNAs targeted to TRAC site 2 and CTLA4 site 9. Panels for sites identified by both GUIDE-seq and CHANGE-seq and classes A and B for TRAC site 2 duplicated from main Fig. 4f for completeness.