Figure 2.

Visualization of Particle Partitioning at AWI Using CryoET

(A) Timescale of grid preparation for tomography samples.

(B–D) Representative tomograms of apoferritin, HSPD1, and ribosome grids prepared at standard blotting speed for Vitrobot (B; time given is Vitrobot “blot time”), (C) fastest time points (TED), and (D) intermediate time points. Green and yellow shaded areas indicate the top and bottom of the AWI, with yellow, gray, and green spheres representing the particle location at the top, middle, and bottom of the ice, respectively. The axis indicates coordinates of the particle location in ice in nanometers. Timescale from sample preparation to vitrification and sample preparation device is shown in the bottom of the box. Full raw data can be seen in Figure S2.