Dissociation of Ribosomal Subunits over Varying Timepoints and Vitrification Devices
(A) Position of ribosomal protein L31 (from PDB: 6OSK) in the 70S ribosome.
(B) Density for L31 in 70S reconstructions compared between all timepoints. While present in “fast” reconstructions, the density is absent in the 200 ms or Vitrobot reconstructions.
(C) Position of ribosomal protein L31 (from PDB: 6OSK) in the 50S ribosome.
(D) Density for L31 in 50S reconstructions compared between all timepoints showing the same trend as in (B).
(E) Position of ribosomal protein S2 in the 30S subunit (from PDB: 6O7K).
(F) Density for S2 in 30S reconstructions compared between all timepoints. The S2 density is missing in the Vitrobot but present in all other reconstructions. 70S in gray, 50S in blue, and 30S in yellow; all maps in (B), (D), and (F) are shown at threshold of 3σ.