REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli BL21 DE3	Sigma Aldrich	69450-M
Chemicals, Peptides, and Recombinant Proteins
Horse Spleen apoferritin	Sigma Aldrich	A3660
E. coli ribosome	New England Biolabs	P0763S
IPTG	Sigma Aldrich	367-93-1
Protease inhibitor cocktail	Calbiochem	539-134
Ni-NTA affinity Resin	Generon	NB-45-00042
10kDa spin concentrator	Vivaspin (Sartorious)	VS0102
16/600 Superdex 200 gel filtration column	GE Healthcare	28-9893-35
Quantifoil 300 mesh CuR 1.2/1.3 grid	Agar Scientific	AGS143-2
Deposited Data
Ribosome 30S (13ms)	From this study	EMDB-10871
Ribosome 30S (54ms)	From this study	EMDB-10872
Ribosome 30S (200ms)	From this study	EMDB-10873
Ribosome 30S (blot)	From this study	EMDB-10874
Ribosome 50S (13ms)	From this study	EMDB-10875
Ribosome 50S (54ms)	From this study	EMDB-10876
Ribosome 50S (200ms)	From this study	EMDB-10877
Ribosome 50S (blot)	From this study	EMDB-10878
Ribosome 70S (13ms)	From this study	EMDB-10879
Ribosome 70S (54ms)	From this study	EMDB-10880
Ribosome 70S (200ms)	From this study	EMDB-10881
Ribosome 70S (blot)	From this study	EMDB-10882
HSPD1 (6ms)	From this study	EMDB-10883
HSPD1 (50ms)	From this study	EMDB-10884
HSPD1 (54ms)	From this study	EMDB-10885
HSPD1 (blot)	From this study	EMDB-10886
Recombinant DNA
HSPD1 plasmid	Dr. Jason Gestwicki & Dr. Hao Shao	N/A
Software and Algorithms
MotionCor2	Zheng et al., 2017	https://emcore.ucsf.edu/ucsf-motioncor2
Imod	Mastronarde, 1997; Kremer et al., 1996	https://bio3d.colorado.edu/imod/
EMAN2	Tang et al., 2007	https://blake.bcm.edu/emanwiki/EMAN2
RELION 3	Zivanov et al., 2018	https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page
GCTF	Zhang, 2016	https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/#gctf
crYOLO	Wagner et al., 2019	http://sphire.mpg.de/wiki/doku.php?id=pipeline:window:cryolo
ChimeraX	Goddard et al., 2018	https://www.cgl.ucsf.edu/chimerax/
Mollweide orientation plot	Naydenova and Russo, 2017	https://www.mrc-lmb.cam.ac.uk/crusso/cryoEF/downloads.html
Other
Vitrobot Mark IV	Thermo Fisher Scientific	N/A
chameleon	SPT Labtech	N/A
TED	Kontziampasis et al., 2019	N/A