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. 2020 Oct 23;3(12):e202000821. doi: 10.26508/lsa.202000821

Figure 4. Ubiquitination promotes secretion of extracellular vesicle (EV)-Cx43.

(A) WB analysis of EV-Cx43 derived from HEK293Cx43+ cells knockdown for Nedd4 (siNedd4), incubated in EV-depleted medium for 8 h (n = 5). (B) Cx43 ubiquitination in HEK293Cx43+ cells evaluated after immunoprecipitation of Cx43. (C) WB analysis of EV-Cx43 derived from HEK293Cx43+ cells knockdown for AMSH (si AMSH) or USP8 (siUSP8), incubated in EV-depleted medium for 8 h (n = 7).

Source data are available for this figure.

Figure 4.

Source Data for Figure 4LSA-2020-00821_SdataF4.pdf (1.9MB, pdf)