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. 2020 Oct 23;3(12):e202000821. doi: 10.26508/lsa.202000821

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© 2020 Martins-Marques et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 6. — (A) Representative transmission electron microscopy of circulating human EVs from control (hEV^CT) and STEMI patients (hEV^STEMI). (B) Representative WB of circulating EVs (30 μg total protein/lane). Heart lysates were used as control. (C) Levels of Cx43 were evaluated in hEV^CT and hEV^STEMI. Individual levels, median, and interquartile range are plotted on graph (n = CT, n = 28 STEMI). (D) WB analysis of Cx43, Alix, Hsp90, and GAPDH in circulating EVs (30 μg total protein/lane). (E) Permeability of EV-Cx43 channels in circulating vesicles from human controls, assessed by sucrose-based transport-specific density shift.

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