Figure 7. Rescue of capillary network formation with supplemental IL-6.
(A) Left: representative images of capillary networks in in vitro 3D angiogenesis assay. Exogenous supplemental γhIL-6 can partially rescue the vascular network formation modulated by N1IC–GFP/HDF. Right: quantitative data of vascular network formation by endothelial cells in 3D angiogenesis assay. Data are analyzed by one-way ANOVA followed by post-hoc test and presented as mean ± SD of three independently performed experiments. (B) Supplemental γhIL-6 in Matrigel reverses the inhibitory effects of N1IC–GFP/HDF on angiogenesis in mouse Matrigel plug model. Left: representative images of IHC. Blood vessels are brown color (DAB) Right: quantitative data of vessel density. Data are analyzed by one-way ANOVA followed by post-hoc test and presented as mean ± SD of five randomly selected fields from each mouse/Matrigel plug in a given group (5 mice/group) (×40).