Figure S5. Dynamic alteration of the Notch pathway activities in fibroblasts presented in mouse non-diabetic wounds versus diabetic skin wounds at various time points.

Wound tissues were harvested at day 1 (early time point), day 7 (middle time point) post-wounding in all mice, and at day 9/13/14/15 (late time point, when wounds are healed) in C57 BL6 (normal non-ischemic wounds)/C57 BL6 (ischemic wounds)/db/db/NOD mice, respectively. Levels of Hes-1 in fibroblasts presented in diabetic wounds (db/db and NOD mice) were detectable at day 1, peaked in day 7, and reduced to a very low level when the wounds were healed in diabetic mice (NOD and db/db), but remained undetectable throughout wound healing process in both normal non-ischemic acute and non-diabetic ischemic chronic wounds (C57 BL6 mice). Immunostaining images show that fibroblasts (green) express higher levels of Hes-1 (red) in wounds of diabetic mice (db/db and NOD) but not in non-diabetic acute wound and ischemic chronic wounds in C57 BL6 mice at day 7. Highlighted areas show fibroblasts in granulation tissues.