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. 2020 Oct 21;9:e50999. doi: 10.7554/eLife.50999

Figure 3. The MT-1303 G1116 GPC variant promotes the formation of VLPs.

Figure 3.

(A) Schematic of C-terminal V5-tagged IbAr10200 or MT-1303 strain GPC constructs. (B) Schematic representation of how the V5-tagged endodomain of CCHFV Gc glycoprotein is protected from proteolytic degradation. (C) Immunoblot analysis of virus-like particles generated by cells transfected with the IbAr10200 or MT-1303 V5-tagged GPC constructs. Closed arrowhead denotes the expected size of the mature CCHFV Gc glycoprotein (75 kDa). Open arrowhead denotes the expected size of the theoretical minimal protected V5 fragment (13.5 kDa). α-tubulin is used as a loading control.