Skip to main content
. 2020 Oct 16;9:e62337. doi: 10.7554/eLife.62337

Figure 3. Genetic architecture and dominance of the Y9 plasmid instability phenotype.

(A) Compared to homozygous BY4742 diploids, heterozygous BY4742/Y9 diploid cells display low plasmid retention after 24 hr, similar to homozygous Y9 diploids. This suggests that the plasmid instability of Y9 cells is a dominant trait. All strains were analyzed with the SCAMPR plasmid retention assay. **p<0.001, ***p<0.0001, Kruskal-Wallis test; n.s. = not significant. (B–C) Phenotype distribution across ~600 random progeny strains (C) shows that most have an intermediate phenotype between that of the parental haploids (B). All strains were analyzed in triplicate with the SCAMPR assay. We selected the bottom ~20% (‘non-permissive’) and top ~20% (‘permissive’) of strains from this distribution for bulk sequencing and segregant analysis.

Figure 3.

Figure 3—figure supplement 1. Tetrads dissected from meiosis of B4742/Y9 heterozygous diploids reveal a range of plasmid stability phenotypes.

Figure 3—figure supplement 1.

All four spores dissected from meiotic tetrads were individually assayed by SCAMPR. While some tetrads display a 2:2 segregation pattern of plasmid instability/stability consistent with a single Mendelian locus, others suggest a more complex inheritance pattern. This pattern is consistent with at least 2–3 independently segregating loci in the Y9 genome that inhibit 2μ plasmid stability. Values plotted are the mean of SCAMPR measurements of three replicates per progeny.