Figure 2. Huaier extract induced S phase accumulation in HepG2 and HuH-6 cells. A. Treatment of HepG2 and HuH-6 cells treated with/without Huaier extract (0, 2, 5 mg/ml) for 24 h was followed by analysis of cell cycle phase distribution, as described in Materials and Methods. Cells were stained with PI for 30 min followed by FACScan flow cytometer analysis. B. HepG2 and HuH-6 cells were incubated in RPMI1640+10% FBS with/without Huaier extract (0, 2, 5 mg/ml) for 24 h. Cyclin D1, Cyclin D3 and actin were detected by western blot. The intensity of Cyclin D1and Cyclin D3 was quantified by ImageJ. After normalization to actin, the fold increase of Cyclin D1and Cyclin D3 versus the control was compared. Data represent mean±SD. *Represents p<0.05.