Figure 4. Effect of Huaier extract on apoptosis in HepG2 and HuH-6 cells. A. HepG2 and HuH-6 cells were incubated in RPMI1640+10% FBS with/without Huaier extract (0, 2, 5 mg/ml) for 48 h. Expression of PARP, cleaved PARP and actin were detected by western blot as described in methods and material. B. The intensity of PARP and cleaved PARP was quantified by ImageJ. After normalization to actin, the fold increase of cleaved PARP versus the control was compared. Data represent mean±SD. *Represents p<0.05.
