Table 3:
Human autograft islet isolation results
| LH | SN | p-Value | |
|---|---|---|---|
| Digestion time (min) | 18.4 ± 4.3 | 22.5 ± 4.3 | 0.0001 |
| Dilution time (min) | 42.5 ± 8.5 | 30.6 ± 7.2 | 0.0001 |
| Percent of digest pancreas weight (%) | 75.6 ± 14.2 | 81.5 ± 11.3 | 0.05 |
| Postdigest IEQ (103) | 222.1 ± 139.1 | 230.4 ± 122.5 | 0.77 |
| Postdigest IEQ/g pancreas | 3642±2330 | 3980± 1924 | 0.46 |
| Postdigest IEQ/islet number | 1.020 ± 0.320 | 0.915 ± 0.291 | 0.07 |
| Postdigested tissue volume (mL) | 13.2 ± 9.9 | 16.6 ± 13.3 | 0.31 |
| No. of purification | 11 (27.5%) | 15 (32.6%) | 0.64 |
| Postpurification IEQ (103) | 216.4 ± 79.3 | 228.0 ± 58.5 | 0.67 |
| Postpurification IEQ/g pancreas (103) | 2566 ± 919 | 2414 ± 1021 | 0.70 |
| Percent recovery (%) | 72.8 ± 25.0 | 72.9 ± 18.7 | 0.99 |
| Postpurification purity (%) | 33.8 ± 20.2 | 34.0 ± 21.5 | 0.87 |
| Postpurification IEQ/islet number | 1.216 ± 0.271 | 1.034 ± 0.219 | 0.10 |
| Embedded islet (%) | 26.8 ± 25.6 | 45.8 ± 26.6 | 0.001 |
| Islet viability (FDA/PI; %) | 91.4 ± 9.8 | 94.1 ± 4.8 | 0.07 |
| Final product volume (mL)/g pancreas | |||
| Digest | 0.20 ± 0.11 | 0.23 ± 0.12 | 0.26 |
| Purified | 0.21 ± 0.09 | 0.25 ± 0.13 | 0.46 |
| Transplanted IEQ/kg body weight | 3142±2191 | 3241 ± 1637 | 0.81 |
LH = Liberase-HI; SN = SERVA/Nordmark; IEQ: islet equivalent. Values are means ± SD.
Percent of digest pancreas weight: (processed pancreas weight − undigested pancreas weight)/(processed pancreas weight) × 100.
Percent recovery: postpurification IEQ/postdigest IEQ × 100. FDA = fluorescein diacetate; PI = propidium iodide.