Figure 3.

P. lentiscus hydrosol reduces p65/NF-κB and CIC expression. U937 cells were treated with P.l 1 : 10 or P.l 1 : 100 for 1 hour and then activated with LPS. Cell lysates, obtained also from untreated cells (C) and cells triggered only with LPS (LPS), were used to evaluate the expression levels of p65/NF-κB (a) and CIC (b). p65/NF-κB, CIC, and β-actin were detected by specific antibodies, and Western blotting images (upper panel (a, b)) are representative of two independent experiments with similar results. The intensities of immunolabeled protein bands were measured by a quantitative software and normalized to β-actin (lower panel (a, b)). Bar charts reported means ± S.D. of p65/NF-κB (a) and CIC (b) protein levels obtained from the abovementioned two independent experiments. Different letters indicate significant differences between treatments at p < 0.05 (Tukey's test).