Fig. 5.

Silencing of SURF-4 in cardiomyocytes affects PCSK9 release. a Expression of SURF-4 in liver and cardiomyocytes (CMC) of adult Wistar rats. Threshold cycle (Ct) of SURF-4 amplification, normalized to the mean Ct of B2M-amplification (ΔCt). n = 3 (liver) and n = 10 (CMC). b Adult ventricular cardiomyocytes were cultivated under serum-free conditions for 24 h in the presence of scrambled RNA (n = 4) or siRNA targeted against SURF-4 (n = 5). PCSK9 release from cardiomyocytes was analyzed by ELISA and normalized to controls (scRNA). Statistical analysis was performed by unpaired t test. *p ≤ 0.05. Data are mean ± SD