FIGURE 1.

ATF3 is dispensable for steady-state hematopoiesis (A) Representative flow cytometric plots (left) and absolute numbers (right) of LSKs (lineage^–Sca-1⁺C-Kit⁺), LT-HSCs (CD150⁺CD48^–LSKs), ST-HSCs (CD150^–CD48^–LSKs), MPP2 (CD150⁺CD48⁺LSKs), and MPP3/4 (CD150^–CD48⁺LSKs) in the BM cells of Atf3^fl/fl and Atf3^fl/fl Vav1Cre mice (n = 6). (B) Representative flow cytometric plots and percentages of cell cycle distribution of LT-HSCs (CD150⁺CD48^–LSK) in the BM cells of Atf3^fl/fl and Atf3^fl/flVav1Cre mice (n = 6). (C) Absolute numbers of GMPs (CD34⁺CD16/32⁺Lineage^–C-Kit⁺Sca-1^–), CMPs (CD34⁺CD16/32^–Lineage^–C-Kit⁺Sca-1^–), MEPs (CD34^–CD16/32^–Lineage^–C-Kit⁺Sca-1^–), and CLPs (CD127⁺Flk2⁺Lineage^–Sca-1^lowC-Kit^low) in the BM cells of Atf3^fl/fl and Atf3^fl/flVav1Cre mice (n = 6). (D) Complete blood cell counts including WBC, RBC, Plt, and Hb from Atf3^fl/fl and Atf3^fl/flVav1Cre mice (n = 6). Data are representative of two independent experiments. Error bars show mean ± SD.