Fig. 3.

Representative box-plot for relative antibody values across applied antigens, IgG study. A. D4 B. D5 C. HUV; Negative and positive control concentrations were determined to be 81 ​nM and 28 ​nM (D4); 73 ​nM and 15 ​nM (D5); 87 ​nM and 40 ​nM (HUV). The negative and positive control results are shown on each graph as blue and red crosses, respectively. Human monoclonal anti-dsDNA and anti-gp120 Abs were used as a positive and negative controls, respectively. For conversion of A450 to Ab amounts, see calibration curves given in Supplementary Fig. 1. Cut-off value for the positive result is shown as a dashed orange line. For complete results of the study, see Supplementary Table 2.