Fig. 4.

Affinity chromatography and analyses of immunoglobulins from NPSLE and SLE sera. A) General scheme for affinity chromatography using synthetic dsDNA antigens: i) antigen immobilization; ii) incubation with sera; iii) immunoglobulin elution. NHS = N-hydroxysuccinimide; B) Representative elution profile followed by Bradford assay; C-D) ELISA of obtained Abs using D4 and D5 pre-coated plates. In Figure legends, the name of the Ab sample is given for the patient no. applied and the antigen used for the purification.