Figure 4.
Chronically administered electrically stimulated muscle contractions increase number of new astrocytes but not neurons in the DG. (A) Representative section through the DG triple labeled for BrdU (green), NeuN (red), and S100β (blue). The top arrow points to a new neuron, displaying both the green and red label. The bottom arrow points to a new astrocyte, displaying both the green and blue labels. (B) Proportion of BrdU cells co-labeled with S100β astrocyte marker in the DG. (C) Proportion of BrdU cells co-labeled with NeuN mature neuron marker in the DG. (D) Mean total number of new (BrdU-labeled) astrocytes in the DG. (E) Mean total number of new (BrdU-labeled) neurons in the DG. Standard error bars shown; n = 17 per bar. * indicates e-stim group is significantly different from sham (P < 0.05), ** indicates P < 0.0001.