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. 2020 Nov 9;10:19319. doi: 10.1038/s41598-020-76356-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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No chronic effect of electrically stimulated muscle contractions on the number of immature neurons or acute effect on cell proliferation in the DG. (A, B) Representative sections through the DG stained for doublecortin (Dcx), an immature neuronal marker, from a mouse in the chronic sham and e-stim groups, respectively (Experiment 3). (C) Mean total number of Dcx positive cells in the DG shown separately for sham and e-stim groups. (D) Mean volume of the DG. (E, F) Representative sections through the DG stained for Ki67, a marker indicating the cell is undergoing mitosis, from a mouse in the acute sham and e-stim groups, respectively (Experiment 1). (G) Mean total number of Ki67 positive cells in the DG. (H) Mean volume of the DG. Standard error bars shown; n = 16 or 17 per bar.