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. 2020 Nov 9;10:19345. doi: 10.1038/s41598-020-76147-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Neuronal Apoptosis in Neonatal Mice following Exposure to Isoflurane or Sevoflurane. (a, b, d, f) Brain homogenates from neonatal mice sacrificed at 0 h and 4 h after exposure to anesthesia were analyzed by western blotting using antibodies against cleaved caspase-3, caspase-3, cleaved PARP, PARP, cleaved caspase-9, caspase-9, cleaved caspase-12, caspase-12 and β-actin. Full-length blots are presented in Supplementary Fig. 3. (c) Representative immunofluorescence staining of the brain cortex from three groups immediately after treatment (scale bar, 50 μm). (e, g) Densitometric quantification of western blots after normalization to β-actin levels. Values are presented as mean ± SD. *P < 0.05 vs. Control. n = 5 per group. Ctr control, iso isoflurane, sev sevoflurane.