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. 2020 Nov 9;3:656. doi: 10.1038/s42003-020-01377-3

Fig. 3. De novo transcriptome annotation incorporating full-length cDNA sequencing results in longer and more complete annotations.

Fig. 3

a Example of an improved and now full-length gene model. HCON_00001100, a nuclear hormone receptor family member orthologous to C. elegans nhr-85, was incomplete and fragmented in the V1 assembly (multiple colours representing CDSs from three distinct V1 annotations, HCOI00573500, HCOI00573600, and HCOI00573700). The full-length gene model, consisting of six exons (black boxes) flanked by UTR sequences (white boxes) is supported by full-length cDNA Iso-Seq sequences (blue bars). b Comparison of mean length and abundance of annotation features between H. contortus V1 (square), H. contortus V4 (cross), H. contortus McMaster (triangle) and C. elegans (circle) transcriptome annotations.