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. 2020 Nov 9;10:19350. doi: 10.1038/s41598-020-76545-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Principal component analysis for sex determination acquired from variations in the data set. A comparison of data was made between immature and mature dugong subjects, analyzed with only parameter (a), only index (b), and combined parameter and index (c). Data of immature dugong subjects were analyzed using only parameter (d), only index (e) and combined parameter and index (f). Data of mature dugong subjects were collected and analyzed with only parameter (g), only index (h) and combined parameter and index (i). Male subjects are indicated by blue color and female subjects are indicated by pink.