Fig. 1. Overview of the study design.

Study patients received infusion of antibody–dye conjugate with or without a loading dose of unlabeled antibody. One to five days post infusion, patients underwent surgical tumor resection. Fresh tissue samples obtained from the primary tumor were homogenized to quantify antibody–dye concentration in the tissue. Whole-tumor specimen were then formalin-fixed and cut into 5 mm thick tissue sections for macroscopic imaging. Subsequently, 5 µm histological slides were prepared from each tissue paraffin block made from the 5 mm tissue sections for microscopic imaging. Antibody uptake and distribution were measured from tissue homogenates, macroscopic, and microscopic imaging. Some elements of this figure were created with BioRender.com.