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. 2020 Nov 9;10:19361. doi: 10.1038/s41598-020-75777-0

Figure 2.

Figure 2

Effect of Kiss1 on the expression of dopamine-related genes in different brain regions. (A) Schematic sagittal view of a zebrafish brain illustrating the macro-dissected brain regions that were subjected to analyses of gene-expression levels. Blue-shaded regions indicate the distribution of dopaminergic cell populations. (BD) Relative expression (fold-change) of th1 (B), th2 (C) and dat (D) mRNAs (n = 10 fish/group) in macro-dissected brain regions 24-h after central administration of either vehicle (control; distilled water) or Kiss1 (K1) with doses of 10–12 or 10–9 mol/ fish. Data presented as mean ± SD. *P < 0.05; ***P < 0.0001 using two-way ANOVA, Tukey's multiple comparisons test.