Figure 2.
Effect of Kiss1 on the expression of dopamine-related genes in different brain regions. (A) Schematic sagittal view of a zebrafish brain illustrating the macro-dissected brain regions that were subjected to analyses of gene-expression levels. Blue-shaded regions indicate the distribution of dopaminergic cell populations. (B–D) Relative expression (fold-change) of th1 (B), th2 (C) and dat (D) mRNAs (n = 10 fish/group) in macro-dissected brain regions 24-h after central administration of either vehicle (control; distilled water) or Kiss1 (K1) with doses of 10–12 or 10–9 mol/ fish. Data presented as mean ± SD. *P < 0.05; ***P < 0.0001 using two-way ANOVA, Tukey's multiple comparisons test.