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. 2020 Nov 9;10:19361. doi: 10.1038/s41598-020-75777-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of npas4a gene in the brain of transgenic dat:EGFP fish followed by 30-min post Kiss1 administration. (a) Schematic sagittal view of a zebrafish brain illustrating the distribution of dopaminergic cell population (blue-shaded zones) in the brain of zebrafish. Boxes with red indicate the boundaries of the photomicrographic illustrations in below (b–e). (b–e) EGFP (green)-labelled dopaminergic neurons in the olfactory bulb (OB, b); dorsal nucleus of ventral telencephalic area (Vd, c); anterior part of the parvocellular preoptic nucleus (PPa, d) and in the anterior thalamic nucleus (A, e) above the fasciculus retroflexus (FR). Although cells expressing npas4a mRNA (magenta) were seen in several brain regions (b'–e'), there was no expression of npas4a in dopaminergic cells (b"−e"). Scale bars 20 µm.