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. 2020 Oct 27;14:559077. doi: 10.3389/fnins.2020.559077

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Copyright © 2020 Luo, Cai, Qin, Yang, Shu, Liu, Zhang, Zhang, Zhou, Yu and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of the hM3Dq receptors in basal forebrain (BF) cholinergic neurons and activation of neurons by Clozapine N-oxide (CNO). (A) Bilateral injections of AAV-DIO-hM3Dq-mCherry virus and the expression of hM3Dq-mcherry in BF cholinergic neurons (ChAT positive) (scale bar: 100 μm). (B) C-fos expression in cholinergic neurons after hM3Dq mice received CNO or saline intraperitoneal (i.p.) injection (scale bar: 100 μm). (C) Quantification of the proportion of mCherry-positive neurons expressing c-Fos under saline and CNO administration [p < 0.0001, n = 6 (1–2 sections per mouse), unpaired t-test]. ****p < 0.0001.