TABLE 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics | Source or references |
| E. coli | ||
| DH5α | F– Φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rK–, mK+) deoR thi-1 phoA supE44λ– gyrA96 relA1; strain used for cloning procedures | (Hanahan, 1983) |
| BL21(DE3) | F- ompT hsdSB (rB-, mB-) gal dcm (DE3); host for protein production | (Studier and Moffatt, 1986) |
| C. glutamicum | ||
| ATCC 13032 (Cg WT) | Biotin-auxotrophic wild type | (Kinoshita et al., 1957) |
| ΔgabR | WT derivative with an in-frame deletion of gabR (cg0565) | This work |
| Δgdh (LNΔGDH) | ATCC 13032 with deletion of gdh (cg2280) | (Müller et al., 2006) |
| ΔglnA (LNΔGS) | ATCC 13032 with deletion of glnA (cg2429) | (Müller et al., 2006) |
| ΔgltBD (LNΔgltBD) | ATCC 13032 with deletion of gltB (cg0229) and gltD (cg0230) | (Rehm et al., 2010) |
| ΔglnAΔglnA2Δgdh (DA-2) | ATCC 13032 with deletion of gdh (cg2280), glnA (cg2429) and glnA2 (cg2477) | (Walter et al., 2008) |
| Plasmids | ||
| pK19mobsacB | KanR; plasmid for allelic exchange in C. glutamicum; (pK18 oriVE.c., sacB, lacZα) | (Schäfer et al., 1994) |
| pK19mobsacB-ΔgabR | KanR.; pK19mobsacB derivative containing a PCR product covering the up- and downstream regions of gabR (cg0565) | This work |
| pAN6 | KanR; C. glutamicum/E. coli shuttle vector for regulated gene expression using the Ptac promoter, derivative of pEKEx2. | (Frunzke et al., 2008) |
| pAN6-gabR | KanR; pAN6 derivative for expression of gabR (cg0565) under control of Ptac | This work |
| pAN6-gabTDP | KanR; pAN6 derivative for expression of gabTDP cluster | This work |
| pET-TEV | KanR; pET28b derivative for overexpression of genes in E. coli, adding an N-terminal decahistdine tag and a TEV protease cleavage site to the target protein (pBR322 oriVE.c., PT7, lacI) | (Bussmann et al., 2010) |
| pET-TEV-gabR | KanR; pET-TEV derivative for overproduction of GabR (cg0565) | This work |
| pJC1-Ptac-eyfp | KanR; pJC1 derivative containing the eyfp gene under the control of Ptac | (Kortmann et al., 2015) |
| pJC1-venus-term | KanR; E. coli-C. glutamicum shuttle vector, pJC1 derivative carrying the venus coding sequence and additional terminators | (Baumgart et al., 2013) |
| pJC1-PgabT-eYFP | KanR; pJC1-venus-term derivative carrying the promoter of gabT (cg0566) fused to eyfp | This work |
| pJM0462 | CmR; pASK-IBA-3C derivative with the coding sequence of gabT (cg0566, NCgl0462) | (Marienhagen et al., 2005) |