TABLE 1.

AOR, Adh, and Aldh cell free extract specific activity.

	BV AOR	NAD+ ADH	NADP+ ADH	NAD+ ALDH	NADP+ ALDH
WT	0.09 (0.00)	0.01 (0.01)	0.03 (0.01)	0.01 (0.01)	0.01 (0.01)
pta	0.38 (0.02)	0.01 (0.01)	0.00 (0.00)	0.46 (0.01)	0.01 (0.01)
pta aor2	0.47 (0.02)	ND	ND	ND	ND
pta aor2 aor1	0.31 (0.02)	ND	ND	ND	ND
pta adhE1 adhE2	ND	0.03 (0.01)	0.01 (0.01)	0.08 (0.01)	0.01 (0.01)

Cell free extract activities of acetaldehyde ferredoxin oxidoreductase, alcohol dehydrogenase, and aldehyde dehydrogenase, reported in μmol min^–1 mg^–1, measured as reduction of the electron acceptor. The top line lists the electron acceptor, either NAD(P)⁺ or benzyl viologen (BV). AOR, aldehyde ferredoxin oxidoreductase; ADH, alcohol dehydrogenase; ALDH, aldehyde dehydrogenase; ND, not determined. Standard deviation is in parenthesis. For all assays, n = 3.