Fig. 6. FABP3 inhibition alleviated ER stress.
a, b Immunoblot analysis (left) and quantification (right) of the indicated proteins and puromycin incorporation in FABP3-knockdown aged and control muscles (a) and FABP3-knockdown myotubes (b). GAPDH was used as a loading control. TA muscles of aged mice (a) were infected with Ad-shFABP3 virus or Ad-shControl. Mice were injected intraperitoneally with puromycin (0.04 mmol/kg) 5 days after infection (n = 3 mice per group). C2C12 myotubes (b, c) were transfected with siFABP3 or siControl and treated with palmitate (500 μM) or vehicle for 12 h. Myotubes were then incubated with puromycin (1 μM) for 30 min (n = 3 independent experiments) (b). c Time course fluorescence recovery. Note the t1/2 values in FRAP analyses. Black, siControl, n = 6; gray, siFABP3, n = 6; deep blue, siControl + palmitate, n = 6; light blue, siFABP3+palmitate, n = 7 each myotube. Data are presented as means ± S.E.M. Two-tailed unpaired Student’s t-test was used. Source data are provided as a Source Data file.