Effect of temperature on A) R. mucilaginosa CBMAI 1528 dry biomass concentration (g/L) and B) extracellular protease activity (U/mL). The strain was cultured at 10, 15, 20, 25, 30 and 35 °C in 250 mL Erlenmeyer flaks with Sabouraud dextrose medium, and samples were collected every 24 h to perform both determinations. The data are expressed as mean values ± standard deviations of three independent assays.