Fig. 5.
Time course of a batch culture of R. mucilaginosa CBMAI 1528 in bioreactor. The strain was cultured on medium containing 20 g/L glucose and 10 g/L of casein tryptone for 24 h at 20 °C, pH 5.6, 200 vvm, 500 rpm and KLa =92 h−1, with an initial dry biomass concentration of 1 g/L. Aliquots from this culture were taken every hour to determine protease activity, dry biomass and glucose concentration. (●) Dry biomass concentration (g/L); (○) glucose concentration (g/L); (●) protease activity (U/mL).