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. 2020 Oct 22;28:e00546. doi: 10.1016/j.btre.2020.e00546

Table 1.

Factor levels of the 25−1 fractional experimental design used for the optimization of protease production by R. mucilaginosa CBMAI 1528 using different organic nitrogen sources.

Variable Symbol parameter Level
Low (-1) High (+1)
Casein tryptone (g/L) X1 0 2.5
Peptone from animal tissue (g/L) X2 0 2.5
Yeast extract (g/L) X3 0 2.5
Casamino acids (g/L) X4 0 2.5
Peptone from gelatin (g/L) X5 0 2.5