Table 3.
Composition of the different culture media tested to optimize protease production by R. mucilaginosa CBMAI 1528.
| Medium | Glucose (g/L) | Yeast extract (g/L) | Casein tryptone (g/L) | C:N ratio |
|---|---|---|---|---|
| 1 | 20 | 0 | 10 | 9.72 |
| 2 | 20 | 5 | 5 | 10.25 |
| 3 | 20 | 10 | 0 | 10.87 |
| 4 | 30 | 0 | 15 | 9.72 |
| 5 | 30 | 7.5 | 7.5 | 10.25 |
| 6 | 30 | 15 | 0 | 10.87 |
| 7 | 40 | 0 | 20 | 9.72 |
| 8 | 40 | 10 | 10 | 10.25 |
| 9 | 40 | 20 | 0 | 10.87 |
The strain was grown on different media at 20 °C, pH 5.6, in flasks shaken at 250 rpm for 48 h. Aliquots from these cultures were taken to determine protease activity and biomass concentration.