Table 5.

Inhibitor reactivity.

Inhibitor	Specificity	Solvent	Inhibitor concentration	Residual activity (%)a
None				100.0
AEBSFb	Serine proteases	Water	1 mM	87.4
6- Aminohexanoic acid	Chymotrypsin, Factor VIIa, lysine carboxypeptidase, plasmin, and plasminogen activator	Water	40 mM	94.8
Antipain hydrochloride	Serine proteases	Water	0.1 mM	93.5
Aprotinin	Serine proteases	Water	0.1 mM	74.8
Benzamidine hydrochloride hydrate	Trypsin, trypsin-like enzymes, and serine proteases	Water	4 mM	91.6
Bestatin	Metalloproteases	Water	0.04 mM	100.0
Chymostatin	Chymotrypsin	DMSOc	0.1 mM	46.0
E-64	Cysteine proteases	Water	10 μM	94.2
EDTA	Metalloproteases	Water	1 mM	86.6
N-Ethylmaleimide	Cysteine proteases	Ethanol	1 mM	69.8
Leupeptin	Serine and Cysteine proteases	Water	0,1 mM	68.7
Pepstatin	Aspartic proteases	Ethanol	1 μM	4.9
Phosphoaramidon	Metalloproteases	Water	10 μM	86.4
Trypsin inhibitor	Trypsin	Water	1:1 stoichiometric binding	81.2

^aEnzyme samples were incubated with the inhibitor for 15 min at 20 °C and then the remaining activity in the sample was determined. Each value represents the mean of three experiments. The activity in the absence of inhibitor was considered 100 %.

^bAEBSF 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride.

^cDimethyl Sulfoxide (DMSO).