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Effect of fat layer removal step. After the first centrifugation step, the fat layer was removed. Code 0=No additional step, 1=Additional step of fat removal. Boxplots show that a) 260/280 ratio undergoes no significant improvement (p = 0.14); b) 260/230 ratio has significant improvement with the additional step (p<0.001).
* p<0.05 (Two sample Wilcoxon rank sum test with continuity correction).
