Table 1.
The comparison of RNA yield (ng/mg), A260/280 and A260/230 in samples extracted by different volumes of TRIzol reagent (expressed as median and Q1-Q3; Q1: 1st quartile, Q3: 3rd quartile).
| Group | TRIzol volume (μL) per unit mass of tissue (mg) | No. of samples (n=) | Yield (ng/mg)* | 260/280 ratio | 260/230 ratio* |
|---|---|---|---|---|---|
| 1 | 4:1 | 4 | 5.60 (4.56–6.71) | 1.74 (1.65–1.86) | 0.20 (0.16–0.52) |
| 2 | 2:1 | 5 | 13.30 (11.20–17.60) | 2.01 (1.69–2.04) | 0.60 (0.29–1.03) |
| 3 | 1:1 | 5 | 25.80 (21.65–30.95) | 1.91 (1.77–2.04) | 0.97 (0.96–1.27) |
| 4 | 2:3 | 16 | 25.35 (17.04–31.06) | 1.97 (1.92–2.02) | 1.22 (1.16–1.68) |
| 5 | 1:2 | 22 | 37.93 (28.99–49.50)a | 1.95 (1.89–1.99) | 1.79 (1.72–1.96)b |
*p<0.001 (Kruskal-Wallis rank sum test).
Post-hoc analysis by pairwise comparisons using Wilcoxon rank sum test revealed:.
a
adjusted p<0.05 with groups 1, 2 and 4.
b
adjusted p<0.05 with all other groups.