Table 2.
The comparison of RNA yield (in both ng/μL and ng/mg), A260/280 and A260/230 in samples extracted using combination of different tissue amounts and TRIzol volumes (expressed as median and Q1-Q3).
| Groups | Tissue mass (mg); TRIzol volume (μL) | n= | Yield (ng/μL)* | Yield (ng/mg) [according to tissue mass] | Yield (ng/mg)* | 260/280 ratio* | 260/230 ratio* |
|---|---|---|---|---|---|---|---|
| Group1 | 200; 2:3 | 6 | 101.03 (81.5–105.1) | 26.19 (20.4–28.1) | 25.28 (20.4–26.3) | 1.97 (1.88–2.07) | 1.20 (0.73–1.33) |
| Group2 | 200; 1:2 | 4 | 111.24 (97.6–147.0) | 27.83 (24.4–36.8) | 1.93 (1.87–1.97) | 1.72 (1.55–1.77) | |
| Group3 | 300; 2:3 | 6 | 178.72 (154.2–188.4) | 29.79 (27.5–33.5) | 29.78 (25.7–31.4) | 1.98 (1.95–2.01) | 1.72 (1.60–1.88)i |
| Group4 | 300; 1:2 | 2 | 203.49 (187.0–220.0) | 33.93 (31.2–36.7) | 2.02 (2.01–2.03) | 1.84 (1.77–1.90) | |
| Group5 | 400; 2:3 | 2 | 209.48 (189.9–229.1) | 35.89 (30.7–48.4) | 20.63 (13.4–27.9) | 2.09 (2.05–2.11) | 1.49 (1.28–1.69) |
| Group6 | 400; 1:2 | 8 | 328.37 (265.9–411.7)a | 41.05 (33.2–51.5)d | 1.88 (1.85–1.92)g | 1.84 (1.77–2.11)j | |
| Group7 | 500; 2:3 | 2 | 133.27 (113.8–152.8)b | 37.94 (30.2–48.0) | 13.33 (11.4–15.3)e | 1.97 (1.95–1.98) | 1.17 (1.16–1.18)k |
| Group8 | 500; 1:2 | 8 | 417.83 (375.2–492.1)c | 41.78 (37.5–49.2)f | 1.98 (1.96–1.99)h | 1.84 (1.79–1.96)l |
*p<0.05 (Kruskal-Wallis rank sum test).
The ng RNA per tissue is presented for each of the four groups of initial tissue amounts
Post-hoc analysis by pairwise comparisons using Wilcoxon rank sum test revealed significant differences (p<0.05) between:.
RNA yield of Group6 vs. Group1 (p = 0.001), Group6 vs. Group2 (p = 0.016), and Group6 vs. Group3 (p = 0.003).
RNA yield of Group7 vs. Group6 (p = 0.044).
RNA yield of Group8 vs. Group1 (p = 0.001), Group8 vs. Group2 (p = 0.004), Group8 vs. Group3 (p = 0.001), Group8 vs. Group4 (p = 0.044), Group8 vs. Group7 (p = 0.044).
Yield (ng/mg) of Group6 vs. Group1 (p = 0.016).
Yield (ng/mg) of Group7 vs. Group6 (p = 0.014).
Yield (ng/mg) of Group8 vs. Group1 (p = 0.008), Group8 vs. Group7 (p = 0.009).
260/280 ratio of Group6 vs. Group3 (p = 0.017), Group6 vs. Group4 (p = 0.049), and Group6 vs. Group5 (p = 0.049).
260/280 ratio of Group8 vs. Group6 (p = 0.003).
260/230 ratio of Group3 vs. Group1 (p = 0.041).
260/230 ratio of Group6 vs. Group1 (p = 0.006).
260/230 ratio of Group7 vs. Group6 (p = 0.049).
260/230 ratio of Group8 vs. Group1 (p = 0.001), Group8 vs. Group2 (p = 0.049), and Group8 vs. Group7 (p = 0.044).