Table 4.
Ct values (average) for GAPDH and SMAD7, and RNU6 and miR181 in five samples (n = 5) comparing our standardised protocol with the unoptimised protocol.
| Sample | Without optimisation |
With optimisation |
||||||
|---|---|---|---|---|---|---|---|---|
| GAPDH | SMAD7 | RNU6 | miR181 | GAPDH | SMAD7 | RNU6 | miR181 | |
| 1 | 23.21 | 25.70 | 20.34 | 26.12 | 21.33 | 23.87 | 18.61 | 24.40 |
| 2 | 23.26 | 23.70 | 22.97 | 30.01 | 21.27 | 23.62 | 21.19 | 27.17 |
| 3 | 22.15 | 25.15 | 23.08 | 29.05 | 19.33 | 22.43 | 20.97 | 25.09 |
| 4 | 20.62 | 24.17 | 21.88 | 28.26 | 20.69 | 24.17 | 19.19 | 24.90 |
| 5 | 24.62 | 23.75 | 24.52 | 29.90 | 22.43 | 25.38 | 21.34 | 26.82 |
| CV(%) | 6.5 | 4.8 | 6.9 | 5.5 | 5.3 | 4.4 | 6.2 | 4.8 |
| p-value | 0.07 | 0.36 | 0.03* | 0.01* | ||||
*p<0.05 (two sample t-test).
Comparison by two sample t-test revealed significant differences between the Ct values of RNU6 and miR181.