Table 5.
Comparison of RNA yields for adipose tissue RNA extraction protocols by different research groups.
| Type of tissue | Site | Method used | RNA yield (in μg/mg) | 260/280 ratio | 260/230 ratio | Authors |
|---|---|---|---|---|---|---|
| Human adipose tissue | Subcutaneous and omental | TRIzol- 1.5 mL for 100 mg | 0.06 | 1.70 | – | Engeli et al. 1999 [18] |
| Primary human adipocytes | Mammary adipose tissue | TRIzol- 1.5 mL for 1 g or 2 × 105 cells | 0.02 | 1.58 | – | Janke et al. 2001 [3] |
| Porcine adipose tissue | Retroperitoneal | TRI Reagent and miRNeasy (combined) | 0.044 | 2.00 | 1.73 | Cirera 2013 [7] |
| Human adipose tissue | Subcutaneous: abdominal or mammary | RNeasy Lipid Tissue Kit | 0.133 | – | – | Hemmrich et al. 2010 [4] |
| Human adipose tissue | Subcutaneous, perivascular, and epicardial | TRIzol and RNeasy kit | – | 2.09 | 1.95 | Sinitsky et al. 2018 [6] |
| Human adipose tissue | Subcutaneous adipose tissue | MagNA Pure Compact RNA Isolation kit | 0.034 | 1.74 | – | Lacinova et al. 2008 [9] |
| Animal adipose tissue | Subcutaneous adipose tissue | SDS, mercapto-ethanol and guanidium chloride extraction buffer | 0.011–0.052 | – | – | Sharma et al. 2017 [10] |
| Porcine tissues | Liver, muscle, hypophysis, adipose tissue, intestinal mucosa | TRIzol- 1 mL per 100 mg tissue powder | 0.50 | 1.8–2.0 | – | Mendez et al. 2011 [11] |
| Rat adipose tissue | Epididymal and perirenal | Guanidium thiocyanate extraction buffer- 1 mL per 1.5 g tissue | 0.07 | 2.0–2.2 | – | Tavangar et al. 1990 [13] |
| Human adipose tissue | Abdominal visceral | TRIzol- 250μL per 500 mg of tissue | 0.042 | 1.98 | 1.84 | Current study |
Total RNA yields are normalized to μg/mg for each study, wherever data was available.
In case multiple methods were employed, the method with the best overall outcomes have been mentioned.