Figure 2.

Accumulation of T_H17 and T_reg Cells in TACI^−/− Compared with WT Mice

Cells isolated from the Spl, mLN, PP, and LP of TACI^−/− (filled circles) or WT (open circles) mice were stimulated with PMA and ionomycin for 4 h and assessed for intracellular IL-17A (A) and IL-10 (B) expression in CD3⁺CD4⁺ T cells by flow cytometry. Cells from various organs of TACI^−/− or WT mice as in (A and B) were assessed for RORγt (C) and Foxp3 (D) expression in CD3⁺CD4⁺ T cells. Data are based on five or more mice analyzed with each symbol representing one mouse and horizontal bars indicating the mean; Mann-Whitney U test, ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ns, not significant. Actual p values are indicated in parentheses below the asterisks or “ns.”