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. 2020 Oct 22;26:100335. doi: 10.1016/j.jbo.2020.100335

Fig. 2.

Fig. 2

FOXD3-AS1 sponged miR-296-5p in OS. (A) Subcellular fractionation assay measured the distribution of FOXD3-AS1 in OS cells. (B) Ten miRNAs that might bind with FOXD3-AS1 were predicted via starBase. (C) RT-qPCR detected FOXD3-AS1 expression after the transfection of pcDNA3.1/FOXD3-AS1 or pcDNA3.1. (D) Luciferase reporter assay measured the impact of FOXD3-AS1 overexpression on the luciferase activity of reporters covering above ten miRNAs. (E) RT-qPCR determined the expression of miR-296-5p in 52 OS samples and matched non-tumor controls. (F) The binding sequences between miR-296-5p and FOXD3-AS1 were predicted by starBase. (G) RT-qPCR detected miR-296-5p expression in OS cells with the transfection of NC mimics or miR-296-5p mimics. (H) The luciferase activity of FOXD3-AS1-WT or FOXD3-AS1-Mut in different groups was measured by luciferase reporter assay. Above data were all analyzed via Student’s t test. *P < 0.05, **P < 0.01.