Fig. 3.

Overexpression of miR-296-5p hindered OS cell migration, invasion and EMT. (A) The migration of MG-63 and HOS cells with or without miR-296-5p elevation was monitored by wound healing assay (scale bar = 200 μm). (B) Transwell assay (scale bar = 200 μm) tested the invasion of indicated MG-63 and HOS cells. (C) Western blot measured E-cadherin and E-cadherin protein levels in MG-63 and HOS cells after different transfections. GAPDH was the loading control. (D) IF assay (scale bar = 50 μm) evaluated the fluorescence intensity of E-cadherin and N-cadherin in indicated MG-63 and HOS cells. These results were all analyzed via Student’s t test. *P < 0.05, ^**P < 0.01.